| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 87-116 from the human protein was used as the immunogen for this WISP-2 antibody. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
WISP-2 Antibody / WNT1-inducible-signaling pathway protein 2 / CCN5 is an antibody targeting WISP-2, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: WISP-2.
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Purified.
- Species reactivity: Human.
- Listed applications: WB, IHC-P (refer to on-page specifications for application-specific guidance).
Biological background
CCN5, also known as WISP-2 (Wnt-1-induced secreted protein 2), is a member of the CCN family of proteins that are involved in cell growth, differentiation, and migration. It is known for its ability to modulate signaling pathways that regulate various cellular activities, making it a key player in maintaining homeostasis in tissues. One of the primary functions of CCN5 is its role in regulating the growth and development of tissues, particularly in the cardiovascular system. Studies have shown that CCN5 can protect against cardiac fibrosis and hypertrophy, making it a potential therapeutic target for heart-related diseases. Furthermore, CCN5 has been found to have anti-inflammatory properties, making it a promising candidate for the treatment of inflammatory conditions. Its ability to regulate immune responses and modulate cytokine production highlights its potential as a target for managing inflammatory disorders. CCN5 has also been implicated in the regulation of metabolic processes and energy homeostasis. Research has shown that CCN5 can influence lipid metabolism and insulin sensitivity, suggesting its potential use in combating metabolic disorders such as obesity and diabetes.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
