| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Cdkn1a recombinant protein (amino acids M1-Q170) was used as the immunogen for the Zebrafish Cdkn1a antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Cdkn1a Antibody / p21 / Waf1 / Cip1 is a anti-CDKN1A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: CDKN1A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Cdkn1a is expressed in various tissues during embryogenesis and in adult organs, especially in regions undergoing rapid cell division or experiencing genotoxic stress. Zebrafish Cdkn1a is a confirmed ortholog of the human CDKN1A gene, which encodes the protein commonly known as p21. Both zebrafish and human Cdkn1a proteins are structurally conserved and share similar mechanisms of action in regulating the cell cycle and promoting cellular senescence or apoptosis.
Cdkn1a is a downstream effector of the tumor suppressor protein p53 and becomes upregulated following DNA damage or oxidative stress. It functions primarily by binding to and inhibiting cyclin E and cyclin A associated kinases, thereby enforcing a cell cycle arrest at the G one and S phase transition. In zebrafish, Cdkn1a is widely used as a biomarker for DNA damage response and is essential for proper embryonic development, tissue regeneration, and maintenance of genomic stability.
Due to its evolutionary conservation and central role in cell cycle control and stress response, zebrafish Cdkn1a is a valuable model for studying cancer biology, toxicology, aging, and regenerative medicine. It is often used in research investigating p53 signaling, chemical screening, and genetic regulation of cell cycle arrest.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
