| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Etv2 recombinant protein (amino acids M1-I218) was used as the immunogen for the Zebrafish Etv2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Etv2 Antibody / ETS variant transcription factor 2 / Etsrp is a anti-ETV2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: ETV2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Etv2 is expressed early in the lateral plate mesoderm and is one of the first transcription factors to mark cells destined to become vascular endothelial cells and hematopoietic progenitors. It activates a network of downstream genes required for the formation of blood vessels and blood cells, including regulators of endothelial identity and vascular integrity. Etv2 also works in coordination with other transcription factors to initiate and stabilize lineage commitment.
Loss of Etv2 function in zebrafish results in the absence of major blood vessels and blood cells, underscoring its essential role in mesodermal patterning. Because of its early and transient expression during development, zebrafish Etv2 is a valuable tool for studying the genetic control of vasculogenesis, hematopoiesis, and mesodermal differentiation.
Etv2 is also widely used in regenerative medicine and stem cell research as a reprogramming factor capable of inducing endothelial identity. Its functional conservation across species makes zebrafish an ideal model for investigating cardiovascular and blood development, as well as for screening drugs that target vascular and hematopoietic pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
