| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Flt1 recombinant protein (amino acids L32-K1229) was used as the immunogen for the Zebrafish Flt1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Flt1 Antibody / Vegfr1 / Vegf receptor 1 is a anti-FLT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: FLT1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Zebrafish Flt1 is expressed in endothelial cells and is involved in the regulation of blood vessel formation by modulating VEGF signaling. Unlike its counterpart Kdrl (zebrafish VEGFR-2), which promotes angiogenic signaling, Flt1 primarily functions as a decoy receptor that binds VEGF-A and VEGF-B with high affinity, thereby fine-tuning angiogenic responses during embryogenesis. Zebrafish also express a soluble form of Flt1 (sFlt1), which lacks the intracellular kinase domain and acts as a natural inhibitor of VEGF signaling.
Expression of flt1 in zebrafish is detected early during embryonic development, particularly in developing vasculature such as the dorsal aorta, cardinal vein, and intersegmental vessels. Disruption of flt1 leads to aberrant vessel patterning and excessive endothelial sprouting, highlighting its essential regulatory function in vascular morphogenesis.
Due to the optical transparency of zebrafish embryos and the availability of vascular reporter lines, zebrafish Flt1 serves as a powerful model for studying angiogenesis, vascular remodeling, and the mechanisms of VEGF signaling in vivo. It is also a valuable tool for investigating vascular defects, cancer biology, and responses to anti-angiogenic therapies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
