| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Fshb recombinant protein (amino acids E19-H130) was used as the immunogen for the Zebrafish Fshb antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Fshb Antibody / Follicle stimulating hormone beta is a anti-Zebrafish Fshb Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Fshb
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Zebrafish Fshb plays a crucial role in the development and maturation of gametes, particularly in promoting folliculogenesis in females and spermatogonial proliferation in males. Its expression and secretion are primarily regulated by gonadotropin-releasing hormone (GnRH) and modulated by feedback from sex steroids and other endocrine factors within the hypothalamic-pituitary-gonadal (HPG) axis.
During zebrafish development, fshb expression becomes evident post-embryonically and increases with sexual maturation. Functional studies have shown that Fshb is essential for early-stage follicle development and the onset of puberty, while also contributing to basal steroidogenesis in the gonads.
Given the genetic accessibility and transparency of zebrafish, the fshb gene and its protein product are commonly used in reproductive biology, toxicology, and endocrine disruption studies. Zebrafish Fshb shares structural and functional homology with its mammalian counterparts, making it a valuable model for exploring conserved mechanisms of vertebrate reproduction.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
