| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Glucocorticoid receptor recombinant protein (amino acids M1-K746) was used as the immunogen for the Zebrafish Glucocorticoid receptor antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Glucocorticoid receptor Antibody / Gr / Nr3c1 is a anti-NR3C1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: NR3C1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
The zebrafish glucocorticoid receptor is expressed in a wide range of tissues including the brain, liver, muscle, and developing endocrine organs. Upon binding to glucocorticoids, the receptor translocates to the nucleus where it binds specific DNA sequences and modulates the transcription of target genes involved in maintaining homeostasis under stress conditions. It also influences cell differentiation, energy balance, and the inflammatory response.
In zebrafish embryos, activation or disruption of glucocorticoid signaling affects key developmental processes such as axis formation, organ maturation, and behavioral programming. Because zebrafish produce cortisol as their primary glucocorticoid, they serve as a powerful model for investigating hormone signaling and endocrine regulation in vertebrates.
The glucocorticoid receptor in zebrafish is widely used in research on stress physiology, neuroendocrine development, immune modulation, and environmental toxicology. It is also a valuable target in studies examining the effects of synthetic glucocorticoids and endocrine disrupting compounds.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
