| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Hand2 recombinant protein (amino acids M1-Q208) was used as the immunogen for the Zebrafish Hand2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Hand2 Antibody / Heart and neural crest derivatives expressed 2 is a anti-HAND2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: HAND2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
During early zebrafish embryogenesis, Hand2 is expressed in the lateral plate mesoderm and contributes to the specification and differentiation of cardiac progenitor cells. It is particularly important for the formation of the heart tube and for the development of the atrial and ventricular chambers. Hand2 also regulates the migration and function of neural crest cells, which give rise to structures such as the jaw and pharyngeal arches.
Loss of Hand2 function in zebrafish leads to severe defects in heart formation, including impaired looping and chamber development, as well as abnormalities in craniofacial structures. Because of its conserved role in vertebrate development, zebrafish Hand2 is widely used as a model for studying congenital heart defects, craniofacial malformations, and the genetic pathways that guide mesodermal and neural crest cell differentiation.
Zebrafish Hand2 is a valuable tool in developmental biology, regenerative medicine, and disease modeling, particularly in research focused on cardiac development and structural birth defects.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
