| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Kita recombinant protein (amino acids R22-N946) was used as the immunogen for the Zebrafish Kita antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Kita Antibody / Kit / Scfr is a anti-Zebrafish Kita Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Kita
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Zebrafish possess two paralogs of the kit gene due to a teleost-specific genome duplication: kita and kitb. Among them, kita is primarily responsible for the development of melanophores, the pigment cells responsible for black coloration. Mutations in kita, such as the well-characterized sparse mutant, lead to a severe reduction in melanophore number, establishing Kit as a key regulator of pigment cell differentiation and migration.
The Kit receptor is activated by its ligand, Kit ligand (Kitlg or Stem Cell Factor), which initiates downstream signaling through pathways such as MAPK/ERK, PI3K/Akt, and JAK/STAT, promoting cell survival, proliferation, and migration. In addition to its role in pigmentation, zebrafish Kit signaling is implicated in the survival and migration of primordial germ cells (PGCs) and has been suggested to influence early blood cell development.
Zebrafish Kit proteins are functionally conserved with their mammalian counterparts, making the zebrafish model an effective system for investigating stem cell biology, oncogenic receptor tyrosine kinase signaling, and genetic disorders associated with KIT dysfunction, such as piebaldism and certain cancers.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
