| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Lysozyme recombinant protein (amino acids K19-A151) was used as the immunogen for the Zebrafish Lysozyme antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Lysozyme Antibody / Lyz is a anti-Zebrafish Lysozyme Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: Zebrafish Lysozyme
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
Lysozyme functions by cleaving the glycosidic bonds in peptidoglycan, a major structural component of bacterial cell walls, particularly in gram positive bacteria. This activity leads to bacterial lysis and supports the clearance of pathogens from the host. In zebrafish, lysozyme genes are expressed during embryogenesis and are upregulated in response to infection or immune stimulation, making them important markers of immune activation.
Zebrafish lysozyme is highly conserved with its mammalian counterparts and is commonly used in research focused on host pathogen interactions, inflammation, and immune system development. It is also a valuable tool for studying the effects of genetic mutations, environmental exposures, or pharmaceuticals on immune responses in vivo.
Because of the transparency of zebrafish embryos and the availability of immune cell specific transgenic lines, lysozyme expression can be visualized and quantified in real time during infection and inflammation models. This makes zebrafish lysozyme a widely used marker in developmental immunology and disease research.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
