| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Nanos3 recombinant protein (amino acids M1-W159) was used as the immunogen for the Zebrafish Nanos3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Nanos3 Antibody / Nanos homolog 3 is a anti-NANOS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: NANOS3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
Zebrafish Nanos3 is an ortholog of the human NANOS3 gene. Both genes belong to the Nanos family of translational repressors and share conserved structural features, including zinc finger domains that enable binding to target messenger RNAs. Nanos3 acts primarily by interacting with other RNA binding proteins to repress the translation of specific transcripts involved in cell differentiation and apoptosis.
In zebrafish embryos, Nanos3 is expressed maternally and zygotically in germ cell precursors. It ensures the correct localization and survival of these cells as they migrate toward the developing gonads. Loss of Nanos3 function in zebrafish leads to the mislocalization and eventual loss of germ cells, resulting in infertility or defective gonadal development.
Because of its conserved role in germline development, zebrafish Nanos3 serves as a valuable marker for early germ cells and is widely used in studies of reproductive biology, stem cell maintenance, and gene regulation. It is also a useful model for understanding human infertility and germ cell tumors.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
