| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Pdx1 recombinant protein (amino acids M1-S246) was used as the immunogen for the Zebrafish Pdx1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Pdx1 Antibody / Pancreas/duodenum homeobox protein 1 is a anti-PDX1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PDX1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Pdx1 is an ortholog of the human PDX1 gene. Both zebrafish and human Pdx1 proteins share significant sequence similarity and functional conservation, particularly in regulating pancreatic development and insulin gene expression. The similarities between the zebrafish and human versions of Pdx1 make zebrafish an excellent model for studying pancreatic biology, beta-cell development, and diabetes.
During embryogenesis, Pdx1 is highly expressed in the developing pancreas, where it directs the differentiation of pancreatic progenitor cells into insulin-producing beta cells. In zebrafish, Pdx1 is essential for the formation of the pancreatic islets and the regulation of insulin secretion in response to blood glucose levels. Pdx1 also plays a key role in beta-cell regeneration after injury, making it a critical factor in pancreatic plasticity.
Zebrafish Pdx1 is expressed predominantly in the pancreas and duodenum during development and in adult stages. The protein regulates the expression of several downstream genes involved in pancreatic endocrine function and beta-cell maturation, including insulin, glucagon, and somatostatin. Pdx1 also interacts with other transcription factors involved in pancreatic development, such as Ngn3 and Pax4, to coordinate the formation of the pancreatic islet.
Given its essential role in pancreatic development, insulin production, and beta-cell regeneration, zebrafish Pdx1 is an important model for studying type 1 and type 2 diabetes, as well as pancreatic diseases and beta-cell dysfunction. The zebrafish model offers a unique opportunity to study pancreatic regeneration, gene regulation, and metabolic disorders in real time.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
