| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Ppm1g recombinant protein (amino acids E45-S456) was used as the immunogen for the Zebrafish Ppm1g antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Ppm1g Antibody / Protein phosphatase 1G is a anti-PPM1G Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PPM1G
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Ppm1g is an ortholog of the human PPM1G gene. The zebrafish and human proteins show significant sequence conservation, particularly in their phosphatase domain, which is crucial for their catalytic activity. This conservation suggests that Ppm1g performs similar roles in signal transduction and cellular regulation across species, making zebrafish a valuable model for studying protein dephosphorylation, cell cycle control, and signal transduction mechanisms.
Ppm1g in zebrafish may also have isoforms, which can vary in their tissue-specific expression and functional roles. These isoforms allow for dynamic regulation of protein phosphorylation during different developmental stages, cellular responses, and physiological conditions. Isoform diversity can contribute to the fine-tuning of cell signaling pathways, especially during embryonic development and stress responses.
Zebrafish Ppm1g is expressed in several tissues, including the brain, heart, and liver, where it plays a role in cellular homeostasis and the regulation of signal transduction pathways involved in metabolism, growth, and development. Its expression is crucial for maintaining protein phosphorylation levels that ensure proper cell cycle progression and stress responses. Disruptions in Ppm1g function in zebrafish can lead to defects in cellular regulation, development, and stress tolerance, providing a useful model for studying diseases related to protein phosphorylation and cell cycle dysfunction.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
