| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Prox1a recombinant protein (amino acids E51-E739) was used as the immunogen for the Zebrafish Prox1a antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Prox1a Antibody / Prospero homeobox 1a is a anti-PROX1A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: PROX1A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Prox1a is one of 2 paralogs, the other being Prox1b, both arising from a genome duplication event. Prox1a is highly conserved and serves as an ortholog of the human PROX1 gene. The human and zebrafish proteins share conserved domains, including the homeodomain and prospero domain, which are essential for DNA binding and transcriptional regulation. This conservation supports the use of zebrafish as a model for studying human developmental biology and disease mechanisms involving PROX1.
Zebrafish Prox1a has known isoforms resulting from alternative splicing. These isoforms may differ in regulatory or functional capacities and show distinct expression patterns during various stages of development. Isoform diversity allows for specialized roles in different tissues and developmental contexts.
Prox1a is expressed in several key regions during zebrafish embryogenesis, including the central nervous system, retina, liver primordium, and developing lymphatic vessels. It is essential for the development of the liver and the specification of lymphatic endothelial cells, functioning as a master regulator of lymphangiogenesis. Loss of Prox1a function in zebrafish results in defects in organ development and disrupted lymphatic vessel formation.
Given its fundamental roles in organ development and lymphatic specification, zebrafish Prox1a is widely used to study transcriptional regulation, stem cell differentiation, and vascular biology. It is also a valuable model for understanding congenital diseases and cancer progression involving the lymphatic system and liver.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
