| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Prpf18 recombinant protein (amino acids E93-L342) was used as the immunogen for the Zebrafish Prpf18 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Prpf18 Antibody / Pre-mRNA-splicing factor 18 is a anti-PRPF18 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PRPF18
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Prpf18 is an ortholog of the human PRPF18 gene. The zebrafish and human proteins are highly conserved, particularly within domains essential for spliceosomal interactions and catalytic activity. This conservation suggests functional equivalency between species, making zebrafish a relevant model for studying pre-mRNA splicing and its regulation in development and disease.
Zebrafish Prpf18 may have isoforms arising from alternative splicing events, although isoform-specific functions are still under investigation. The presence of multiple isoforms may allow differential regulation of splicing in a tissue-specific or developmental stage-specific manner.
During zebrafish embryogenesis, Prpf18 is broadly expressed, with notable enrichment in rapidly dividing and transcriptionally active tissues such as the neural tube and developing somites. Its expression is crucial for early development, as disruption of splicing machinery can lead to widespread defects in gene regulation, cell cycle control, and differentiation.
Given its central role in RNA splicing, zebrafish Prpf18 is important for studies related to gene expression regulation, developmental biology, and spliceosome-associated disorders. It also serves as a useful model for understanding human genetic diseases caused by splicing defects, including certain neurodegenerative and retinal disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
