| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Prpf31 recombinant protein (amino acids N84-K499) was used as the immunogen for the Zebrafish Prpf31 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Prpf31 Antibody / Prp31 / Pre-mRNA processing factor 31 is a anti-PRPF31 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PRPF31
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Prpf31 is the ortholog of the human PRPF31 gene. The zebrafish and human proteins are highly conserved, especially in functional domains that mediate interactions with other spliceosomal proteins. This conservation suggests that Prpf31 in zebrafish plays equivalent roles in pre-mRNA splicing and is suitable for modeling human splicing-related conditions.
Zebrafish Prpf31 may have multiple isoforms due to alternative splicing. These isoforms can contribute to functional diversity and may exhibit distinct spatial or temporal expression patterns during development. Isoform-specific roles are under ongoing investigation and may offer insights into tissue-specific splicing regulation.
Expression of Prpf31 in zebrafish is detected in tissues with high levels of transcriptional activity, such as the developing eye, brain, and somites. During early embryogenesis, its presence is crucial for the proper expression of genes required for organ development and cell differentiation.
Mutations in the human PRPF31 gene are associated with autosomal dominant retinitis pigmentosa, a degenerative eye disease. Zebrafish models have been used to study the pathogenesis of this condition and to explore therapeutic strategies, due to the gene’s conserved function and retinal expression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
