| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Prpf6 recombinant protein (amino acids Q99-K618) was used as the immunogen for the Zebrafish Prpf6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Prpf6 Antibody / Pre-mRNA processing factor 6 is a anti-PRPF6 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF) with listed reactivity in Zebrafish. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: PRPF6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IF
Biological background
In zebrafish, Prpf6 is an ortholog of the human PRPF6 gene. The zebrafish and human proteins share a high degree of conservation, particularly in domains involved in protein-protein interactions within the spliceosome. This evolutionary conservation supports the use of zebrafish as a reliable model for studying the functional roles of spliceosomal components and for understanding the molecular basis of splicing-related diseases in humans.
Zebrafish Prpf6 may have isoforms generated through alternative splicing. These isoforms may differ in their regulatory features or expression patterns, enabling context-specific or tissue-specific control of splicing events during development or in response to physiological signals.
During embryonic development in zebrafish, Prpf6 is broadly expressed in actively proliferating and transcriptionally active tissues, such as the central nervous system, eye, and somites. Its function is critical for cell viability, as defects in pre-mRNA splicing can result in misexpression of genes required for cell cycle progression, differentiation, and organogenesis.
In humans, mutations in PRPF6 have been linked to retinitis pigmentosa and other splicing-associated disorders. Because of the conserved function and expression of Prpf6 in zebrafish, it serves as a valuable model for investigating the cellular consequences of spliceosomal dysfunction and for testing therapeutic approaches targeting RNA processing defects.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
