| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psma1 recombinant protein (amino acids M1-E250) was used as the immunogen for the Zebrafish Psma1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psma1 Antibody / Proteasome subunit alpha type 1 is a anti-PSMA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PSMA1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Psma1 is the ortholog of the human PSMA1 gene. The zebrafish and human proteins share strong sequence conservation, especially in regions critical for proteasome assembly and function. This conservation highlights the functional equivalence of Psma1 across species and supports the utility of zebrafish as a model organism for investigating proteasome-related pathways and diseases.
Zebrafish Psma1 may have isoforms resulting from alternative splicing. These isoforms could exhibit differences in regulatory sequences or tissue distribution, potentially contributing to specialized roles in specific developmental stages or cell types. However, the primary isoform is typically the dominant functional variant in most tissues.
During zebrafish development, Psma1 is ubiquitously expressed, reflecting the universal need for regulated protein degradation in cellular maintenance. Expression is particularly enriched in proliferative and metabolically active tissues such as the brain, liver, and musculature. The function of Psma1 is critical for embryo viability, as disruption of proteasome function can lead to accumulation of toxic protein aggregates and defects in signaling pathways.
In human biology, proteasome subunit mutations, including those in PSMA1, have been associated with neurodegenerative conditions and immune dysregulation. Because of the high functional conservation, zebrafish Psma1 serves as a powerful tool for modeling these disorders, studying proteasome biology, and evaluating potential pharmacological modulators of proteasome activity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
