| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psma3 recombinant protein (amino acids D84-R130) was used as the immunogen for the Zebrafish Psma3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psma3 Antibody / Proteasome subunit alpha type 3 is a anti-PSMA3 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PSMA3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Psma3 is an ortholog of the human PSMA3 gene. The zebrafish and human proteins are highly conserved in both sequence and structure, especially in domains critical for proteasome assembly and function. This conservation supports the use of zebrafish as a reliable model for studying proteasome dynamics, protein turnover, and diseases related to impaired proteostasis.
Zebrafish Psma3 may exist in multiple isoforms generated through alternative splicing. These isoforms can differ in their regulatory elements, cellular localization, or tissue-specific expression patterns, although the predominant isoform is typically involved in the canonical function of proteasome assembly and protein degradation.
During zebrafish embryogenesis, Psma3 is broadly expressed across many tissue types, reflecting the fundamental role of the proteasome in maintaining cellular protein quality. Expression is particularly high in metabolically active tissues such as the developing brain, eye, liver, and somites. The protein is essential for embryonic growth, tissue morphogenesis, and cellular stress responses.
In human systems, dysfunction of PSMA3 or related proteasome components has been linked to disorders such as cancer, neurodegeneration, and autoinflammatory diseases. Given the high degree of conservation, zebrafish Psma3 is a valuable model for investigating the role of the proteasome in normal physiology and in the pathogenesis of diseases involving impaired protein degradation or ubiquitin-proteasome system dysfunction.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
