| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psma5 recombinant protein (amino acids M1-I241) was used as the immunogen for the Zebrafish Psma5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psma5 Antibody / Proteasome subunit alpha type 5 is a anti-PSMA5 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PSMA5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Psma5 is the ortholog of the human PSMA5 gene. The protein sequence and functional domains are highly conserved between zebrafish and humans, indicating a shared role in proteasome-mediated protein degradation. This evolutionary conservation supports the use of zebrafish as a model organism for studying proteostasis, developmental regulation, and disease mechanisms related to proteasome dysfunction.
Zebrafish Psma5 may have multiple isoforms generated through alternative splicing. These isoforms could allow tissue-specific or developmental stage-specific regulation of proteasome activity, although the canonical isoform is primarily involved in the assembly and function of the standard 20S proteasome.
During zebrafish embryogenesis, Psma5 is broadly expressed in rapidly proliferating and metabolically active tissues, including the developing brain, eye, musculature, and digestive organs. Its expression reflects the universal need for controlled protein degradation in processes such as cell cycle progression, signal transduction, and removal of damaged or misfolded proteins.
In human biology, defects in proteasome components including PSMA5 have been associated with neurodegenerative diseases, cancers, and immune system disorders. Zebrafish Psma5, due to its conserved function, serves as a valuable model for functional studies of the proteasome and for the development of therapeutics targeting the ubiquitin-proteasome system.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
