| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmb1 recombinant protein (amino acids Y18-D237) was used as the immunogen for the Zebrafish Psmb1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmb1 Antibody / Proteasome subunit beta type 1 is a anti-PSMB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PSMB1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Psmb1 is an ortholog of the human PSMB1 gene. The zebrafish and human proteins are highly conserved in sequence and function, indicating evolutionary preservation of this essential proteasome subunit. This makes zebrafish a suitable model organism for investigating proteasome biology and related human diseases.
Zebrafish Psmb1 may have isoforms resulting from alternative splicing, although the predominant isoform is primarily responsible for incorporation into the core proteasome structure. These potential isoforms may contribute to tissue-specific regulation of proteasome function or be expressed under certain developmental or stress conditions.
During zebrafish development, Psmb1 is ubiquitously expressed, reflecting the widespread requirement for protein turnover in cellular homeostasis, growth, and differentiation. Expression is especially prominent in tissues with high metabolic activity, such as the brain, eye, muscle, and liver. Disruption of Psmb1 expression or proteasome assembly can impair embryonic development and lead to cellular stress due to the accumulation of damaged or misfolded proteins.
In humans, alterations in proteasome function including mutations in PSMB1 have been implicated in disorders such as neurodegeneration, cancer, and autoimmune syndromes. Zebrafish Psmb1 is therefore a valuable research target for understanding the roles of the proteasome in development, disease, and therapeutic intervention strategies that modulate proteasomal activity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
