| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmc1 recombinant protein (amino acids K21-L440) was used as the immunogen for the Zebrafish Psmc1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmc1 Antibody / Psmc1a / Psmc1b / 26S proteasome regulatory subunit 4 is a anti-PSMC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PSMC1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Psmc1 is the ortholog of the human PSMC1 gene. The zebrafish and human proteins show strong sequence and functional conservation, especially in the ATPase domain and other motifs critical for proteasome assembly and activity. This high degree of conservation supports the use of zebrafish as a model to study proteasomal function and its relevance to human disease.
Zebrafish Psmc1 may have multiple isoforms generated through alternative splicing. These isoforms can exhibit variation in domain composition or expression patterns and may play specific roles during different stages of development or under stress conditions. However, the canonical isoform is primarily responsible for forming the regulatory ATPase ring of the proteasome.
During zebrafish embryogenesis, Psmc1 is broadly expressed and particularly enriched in rapidly dividing and metabolically active tissues such as the central nervous system, developing musculature, and gastrointestinal organs. Its role in protein quality control is critical for proper cell cycle progression, apoptosis regulation, and differentiation.
In humans, mutations or dysregulation of PSMC1 have been associated with proteasome-related disorders and neurodevelopmental syndromes. Given its conserved function, zebrafish Psmc1 serves as an excellent model for exploring the molecular mechanisms of proteasome activity and its impact on developmental and pathological processes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
