| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmc5 recombinant protein (amino acids N41-K393) was used as the immunogen for the Zebrafish Psmc5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmc5 Antibody / 26S proteasome regulatory subunit 8 is a anti-PSMC5 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PSMC5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
In zebrafish, Psmc5 is the ortholog of the human PSMC5 gene, and they share high sequence identity as well as functional conservation. The zebrafish and human proteins possess conserved domains critical for ATP hydrolysis and substrate processing, making zebrafish an excellent model for studying proteasome function.
Like its human counterpart, Psmc5 in zebrafish may have isoforms resulting from alternative splicing. These isoforms could exhibit differences in regulatory subunit composition or functional roles within the proteasomal complex, especially in specialized tissues or during developmental stages.
Expression of Psmc5 in zebrafish is widespread, with notable presence in actively dividing cells, such as those in the brain, heart, and digestive tissues. This distribution underscores its essential role in development and cellular maintenance.
In humans, mutations or dysregulation of PSMC5 have been associated with various diseases, including neurodegenerative disorders and cancers, often due to impaired proteasomal degradation. Zebrafish Psmc5 serves as an ideal model for studying proteasome-related diseases and for screening potential therapeutic agents that target the proteasome machinery.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
