| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmd13 recombinant protein (amino acids E70-L355) was used as the immunogen for the Zebrafish Psmd13 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmd13 Antibody / 26S proteasome non-ATPase regulatory subunit 13 is a anti-PSMD13 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PSMD13
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Psmd13 is the ortholog of the human PSMD13 gene, sharing high sequence identity and functional conservation between the two species. The zebrafish protein has similar domains responsible for interacting with other proteasomal components and regulatory factors, ensuring proper proteasome assembly and activity.
Like its human counterpart, Psmd13 in zebrafish may have isoforms, likely arising from alternative splicing events. These isoforms may play specialized roles in different tissues or developmental stages, potentially influencing the efficiency of protein degradation in various cellular contexts. Such isoforms could also have tissue-specific expression patterns, contributing to the regulation of cell-cycle progression, stress responses, and signal transduction.
In zebrafish, Psmd13 is highly expressed in tissues with active cell division, such as the brain, liver, and kidney, where proteasomal activity is essential for maintaining protein turnover and cellular integrity. This expression pattern aligns with the protein’s conserved role in protein quality control, cellular differentiation, and apoptosis regulation.
The study of Psmd13 in zebrafish offers valuable insights into proteasome-related diseases in humans, such as neurodegenerative diseases and cancers, where disruptions in proteasomal degradation pathways can lead to the accumulation of damaged proteins. Zebrafish serves as an excellent model for studying the molecular mechanisms of proteasomal dysfunction and for screening potential therapeutic agents aimed at modulating proteasome activity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
