| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmd14 recombinant protein (amino acids D21-H310) was used as the immunogen for the Zebrafish Psmd14 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmd14 Antibody / 26S proteasome non-ATPase regulatory subunit 14 is a anti-PSMD14 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: PSMD14
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
In zebrafish, Psmd14 is an ortholog of the human PSMD14 gene. Both zebrafish and human Psmd14 share substantial sequence conservation, particularly in their role within the 26S proteasome complex, ensuring functional equivalence in protein degradation. This high degree of similarity makes zebrafish a valuable model organism for studying the molecular mechanisms of protein degradation and its impact on various cellular processes, including development, growth, and disease progression.
Psmd14 is expressed in a variety of tissues in zebrafish, including the brain, heart, and muscles, reflecting its importance in general cellular maintenance and metabolic processes. It has a critical role in regulating ubiquitin-mediated proteolysis, which is essential for cell cycle regulation, especially during embryonic development, cell differentiation, and tissue regeneration.
Zebrafish Psmd14 has isoforms, which may vary in their expression patterns and functional properties. These isoforms are likely to have tissue-specific roles, particularly in highly dynamic tissues such as neural and muscle tissue. The presence of isoforms suggests a degree of functional specialization and adaptability to specific cellular contexts, allowing Psmd14 to be involved in diverse aspects of protein homeostasis and stress responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
