| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmd6 recombinant protein.(amino acids T27-Q378) was used as the immunogen for the Zebrafish Psmd6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmd6 Antibody / 26S proteasome non-ATPase regulatory subunit 6 is a anti-PSMD6 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Nuclear, cytoplasmic, extracellular.
Key elements and design rationale
- Target: PSMD6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB, IHC-P
Biological background
In zebrafish, Psmd6 is the ortholog of the human PSMD6 gene, sharing high sequence identity and functional conservation. Both zebrafish and human Psmd6 proteins feature conserved domains that are crucial for their role in proteasome function, allowing for the proper assembly and regulation of the 26S proteasome complex. The similarity between zebrafish Psmd6 and human PSMD6 makes zebrafish an excellent model for studying proteasomal mechanisms and related diseases.
Psmd6 in zebrafish may also have isoforms arising from alternative splicing. These isoforms may exhibit variations in their expression patterns or regulatory functions, which could influence proteasomal activity in different tissues or stages of development. The presence of such isoforms allows for specific regulation of proteasomal function under different cellular conditions, such as during cellular stress, development, or differentiation.
In zebrafish, Psmd6 is expressed in a variety of tissues, particularly in those with high protein turnover, such as the brain, liver, and kidney. This reflects the protein’s essential role in regulating protein degradation and maintaining cellular integrity, particularly in actively dividing cells or tissues under stress.
Due to its functional conservation with human PSMD6, Psmd6 in zebrafish provides an ideal model for investigating proteasomal dysfunctions related to various human diseases, including neurodegenerative disorders, cancer, and immune-related conditions. Disruptions in PSMD6 function can lead to the accumulation of damaged or misfolded proteins, contributing to disease pathogenesis. The zebrafish model facilitates the study of these diseases and offers opportunities for therapeutic screening targeting proteasomal pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
