| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Psmd8 recombinant protein (amino acids M1-V267) was used as the immunogen for the Zebrafish Psmd8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Psmd8 Antibody / 26S proteasome non-ATPase regulatory subunit 8 is a anti-PSMD8 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Zebrafish. Reported localization: Cytoplasmic, nuclear.
Key elements and design rationale
- Target: PSMD8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P
Biological background
In zebrafish, Psmd8 is the ortholog of the human PSMD8 gene, sharing a high degree of sequence conservation and functional similarity. Both the zebrafish and human versions of Psmd8 contain conserved domains required for proteasomal assembly and interaction with other proteasomal subunits, facilitating the proper function of the 26S proteasome complex. The conservation between these species underscores the value of zebrafish as a model organism for studying proteasomal mechanisms and their contribution to human health.
Psmd8 in zebrafish may also have isoforms due to alternative splicing events. These isoforms, while not fully characterized, could exhibit tissue-specific expression or distinct functional roles in different stages of development. Variations in isoforms can allow for the fine-tuning of proteasomal activity in response to different cellular environments, including stress or differentiation signals. The presence of multiple isoforms enhances the adaptability of the proteasome in maintaining cellular balance across various tissues.
In zebrafish, Psmd8 is expressed in several tissues, particularly those involved in high protein turnover and cellular division, such as the brain, liver, and kidney. This broad expression pattern highlights its essential role in controlling the degradation of regulatory proteins and supporting cellular processes like growth, stress responses, and apoptosis.
The functional conservation of Psmd8 between zebrafish and humans makes it an excellent model for understanding the molecular mechanisms underlying proteasomal dysfunctions in humans. Alterations in PSMD8 function have been implicated in various diseases, including neurodegenerative disorders and cancers, where impaired proteasomal degradation leads to the accumulation of toxic proteins. Zebrafish Psmd8 offers a valuable tool for studying these diseases and exploring potential therapeutic strategies aimed at modulating proteasomal activity to treat proteostasis-related disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
