| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Rab5a recombinant protein (amino acids M1-N216) was used as the immunogen for the Zebrafish Rab5a antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Rab5a Antibody / Rab5aa / Rab5ab is a anti-RAB5A Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: RAB5A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Rab5a is an ortholog of the human RAB5A protein and shows a high degree of sequence and functional conservation with its human counterpart. Similar to human RAB5A, zebrafish Rab5a acts as a molecular switch by cycling between an active GTP bound form and an inactive GDP bound form, controlling the recruitment of effectors necessary for vesicle docking and fusion.
In zebrafish, Rab5a exists in 2 isoforms, Rab5aa and Rab5ab. Both isoforms are expressed during embryogenesis and play overlapping but sometimes distinct roles in endosomal dynamics. Rab5aa is often the primary isoform examined in studies of receptor mediated endocytosis, while Rab5ab may have specialized roles in certain tissues or stages of development. The presence of these 2 isoforms provides a unique model to investigate how variations in Rab5 function can impact cellular trafficking pathways.
Antibodies against zebrafish Rab5a protein are valuable for research focusing on vesicle transport, endocytosis, receptor regulation, and developmental biology. The functional similarity between zebrafish Rab5a and human RAB5A also makes this protein an important model for studying conserved mechanisms of intracellular trafficking across species.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
