| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Rbm22 recombinant protein (amino acids D17-R296) was used as the immunogen for the Zebrafish Rbm22 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Rbm22 Antibody / RNA-binding protein 22 is a anti-RBM22 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: RBM22
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Rbm22 is an ortholog of the human RBM22 protein and exhibits a high degree of sequence and functional conservation. In both zebrafish and humans, Rbm22 is involved in regulating alternative splicing events that contribute to the diversity of the transcriptome and the regulation of gene expression. Its function is particularly important during early development when precise control of splicing is required for cell differentiation and tissue formation.
There is currently limited evidence for multiple isoforms of zebrafish Rbm22, although alternative splicing is a common feature of RNA binding proteins, and further studies may reveal additional isoforms in the future. The zebrafish model provides an excellent system to study the conserved roles of Rbm22 in RNA metabolism and developmental processes.
Antibodies targeting zebrafish Rbm22 protein are valuable for research in RNA biology, splicing regulation, and gene expression studies. Given its orthology to human RBM22, studies using zebrafish Rbm22 can provide insights into conserved mechanisms of splicing regulation and its potential links to human disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
