| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Sf3b3 recombinant protein (amino acids R728-E982) was used as the immunogen for the Zebrafish Sf3b3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Sf3b3 Antibody / Splicing factor 3b subunit 3 is a anti-SF3B3 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P, Immunofluorescence (IF) with listed reactivity in Zebrafish. Reported localization: Nuclear.
Key elements and design rationale
- Target: SF3B3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): IHC-P, IF
Biological background
Zebrafish Sf3b3 is an ortholog of the human SF3B3 protein, with strong sequence conservation and similar functional roles. Like its human counterpart, zebrafish Sf3b3 is indispensable for the regulation of alternative splicing, which contributes to transcriptome diversity and precise control of protein synthesis. Disruption of Sf3b3 function can have severe effects on embryonic development and cellular homeostasis due to the widespread impact of splicing errors.
Currently, no distinct isoforms of zebrafish Sf3b3 have been described, but its role as part of the SF3B complex is well established and highly conserved across vertebrate species. The zebrafish model provides a powerful system to study the function of Sf3b3 during development and to investigate splicing defects that may be linked to human diseases.
Antibodies against zebrafish Sf3b3 protein are valuable for studying spliceosome assembly, RNA processing, and transcriptional regulation. These antibodies are commonly applied in techniques such as western blot, immunohistochemistry, immunofluorescence, and ELISA, enabling detailed investigations of splicing factor expression and localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
