| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Sod1 recombinant protein (amino acids M1-Q154) was used as the immunogen for the Zebrafish Sod1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Sod1 Antibody / Superoxide dismutase 1 is a anti-SOD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: SOD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Sod1 is an ortholog of the human SOD1 protein, sharing high sequence and functional conservation. Mutations in human SOD1 are well known for their link to amyotrophic lateral sclerosis (ALS), and zebrafish models expressing mutant Sod1 have been used extensively to study the mechanisms of neurodegeneration and oxidative stress. The conserved enzymatic function of Sod1 in zebrafish makes it a powerful model for investigating oxidative damage, neuronal health, and stress response pathways.
There are no confirmed isoforms of zebrafish Sod1. The protein is expressed broadly in various tissues, with higher expression in metabolically active organs such as the brain, liver, and muscles where reactive oxygen species production is elevated. Its activity is tightly regulated, as both deficient and excessive reactive oxygen species can lead to cellular dysfunction.
Antibodies targeting zebrafish Sod1 protein are important tools for studying oxidative stress pathways, mitochondrial function, and the effects of environmental or chemical stressors on cellular health. These antibodies are commonly used in applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA to analyze Sod1 expression and regulation in zebrafish tissues.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
