| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Spi1b recombinant protein (amino acids M1-M297) was used as the immunogen for the Zebrafish Spi1b antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Spi1b Antibody | Pu.1b | Spi1 is a anti-SPI1B Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: SPI1B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Spi1b is an ortholog of the human SPI1 protein, also known as PU.1, and shares high sequence and functional conservation. Similar to its human counterpart, Spi1b binds to specific ETS motifs in gene promoters and enhancers to activate or repress target genes. It is particularly important in the early stages of myelopoiesis and is a key regulator of macrophage and neutrophil lineage commitment.
The use of a Zebrafish Spi1b antibody allows researchers to examine Spi1b expression and localization in developing blood and immune cells. Such antibodies are essential for applications including western blot, immunohistochemistry, and immunofluorescence, enabling detailed characterization of hematopoietic cell populations. A Zebrafish Spi1b antibody can also be used to investigate transcriptional networks controlling immune development and to study the effects of genetic or chemical perturbations on blood cell formation.
There are no known isoforms of zebrafish Spi1b, and its expression is tightly regulated during embryogenesis and hematopoietic stem cell differentiation. Using a high-quality Zebrafish Spi1b antibody, researchers can gain insights into conserved mechanisms of immune regulation that are relevant to both zebrafish and humans.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
