| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived zebrafish Srp54 recombinant protein (amino acids M1-K433) was used as the immunogen for the Zebrafish Srp54 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Zebrafish Srp54 Antibody / Signal recognition particle 54 kDa protein is a anti-SRP54 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Zebrafish.
Key elements and design rationale
- Target: SRP54
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig
- Format: Antigen affinity purified
- Applications (as listed): WB
Biological background
Zebrafish Srp54 is an ortholog of the human SRP54 protein and exhibits strong sequence and functional conservation. In both zebrafish and humans, Srp54 contains a GTP binding domain and an M domain that recognizes and interacts with hydrophobic signal sequences. These conserved domains highlight the importance of Srp54 in protein translocation processes that are essential for the development and survival of eukaryotic cells.
The use of a Zebrafish Srp54 antibody allows researchers to detect and study the distribution and expression of Srp54 during early development and in various tissues. Such antibodies are valuable for western blot, immunohistochemistry, and immunofluorescence applications, providing insights into the role of the SRP pathway in protein targeting. A Zebrafish Srp54 antibody can also be used to analyze the effects of genetic mutations or experimental treatments on protein translocation and endoplasmic reticulum function.
There are no confirmed isoforms of zebrafish Srp54, and its function appears to be highly conserved across vertebrate species. By using a high-quality Zebrafish Srp54 antibody, researchers can explore the essential roles of the signal recognition particle pathway in protein sorting and its relevance to human biology and disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
