| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SSSCA1/ZNRD2 recombinant protein (Position: Q31-H199) was used as the immunogen for the ZNRD2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ZNRD2 Antibody / Zinc ribbon domain-containing protein 2 / SSSCA1 is a anti-ZNRD2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: ZNRD2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Functionally, ZNRD2 antibody identifies a 179-amino-acid nuclear protein containing a C2H2-type zinc ribbon domain that facilitates nucleic acid and protein interactions. ZNRD2 plays a role in ribosomal RNA synthesis by modulating RNA polymerase I activity within the nucleolus. It interacts with components of the transcriptional machinery, linking chromatin structure to gene expression.
The ZNRD2 gene is located on chromosome 6p21.33 within the major histocompatibility complex (MHC) region, reflecting its immunological relevance. It is expressed in a variety of tissues, including thymus, spleen, and epithelial cells. ZNRD2 has been identified as an autoantigen in autoimmune disorders such as Sjogren syndrome and scleroderma, where antibodies against SSSCA1 epitopes are frequently detected.
Pathologically, aberrant ZNRD2 expression has been associated with cancer and autoimmunity. Its altered transcriptional activity may contribute to abnormal cell proliferation and immune dysregulation. Because ZNRD2 participates in ribosome biogenesis and nuclear organization, its dysfunction can influence cellular growth and stress responses.
ZNRD2 antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence to study nucleolar structure, RNA synthesis, and autoantigen profiles.
Structurally, ZNRD2 consists of an N-terminal zinc ribbon domain responsible for nucleic acid binding and a conserved C-terminal tail that interacts with nuclear proteins. This antibody helps elucidate ZNRD2's functions in chromatin-associated transcription and its role as an autoimmune target in connective tissue diseases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
