| Field | Specification |
|---|---|
| Target | |
| Alternative names | Zyxin|Zyxin-2|ZYX |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | zyxin |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin(ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.
Product Overview
This monoclonal antibody (clone 8D5), raised in Mouse, recognizes ZYX. It is validated for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB | 1:500-1:5000 |
| IHC | 1:20-1:200 |
| IF | 1:20-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
zyxin
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | 8D5 |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Immunogen | zyxin |
| Purification | Protein A+G Purification |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 80 kDa |
| UniProt # | Q15942 |
| Gene ID | 7791 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Zyxin|Zyxin-2|ZYX |
Reactivity Notes
Validated reactivity: Human, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for ZYX research from Fine Test, or contact us for alternative formats.
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