| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Zyxin was used as the immunogen for the Zyxin antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ZYX Antibody / Zyxin is a anti-ZYX Rabbit antibody Recombinant Rabbit Monoclonal clone AFOG-26 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ZYX
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone AFOG-26, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
Zyxin is characterized by three LIM domains at its C-terminus, which mediate protein-protein interactions, and a proline-rich N-terminal region that binds to VASP and alpha-actinin. Through these domains, Zyxin coordinates actin filament assembly and cell-matrix adhesion. The Zyxin antibody supports visualization of these interactions, allowing researchers to examine how this structural adaptor transduces mechanical cues into gene expression changes. Zyxin relocates to the nucleus in response to stress, suggesting a dual cytoplasmic and nuclear role in mechanosignaling.
As a component of focal adhesions, Zyxin participates in processes such as wound healing, embryonic development, and tissue remodeling. It interacts with proteins including LPP, LIMD1, and Ena/VASP to modulate cell shape and movement. The Zyxin antibody supports functional studies of these interactions, revealing how Zyxin integrates extracellular matrix forces with actin cytoskeletal organization. Its recruitment to sites of mechanical strain makes it a critical sensor for cellular adaptation to physical stress.
Altered Zyxin expression has been associated with several diseases, including cancer, cardiovascular dysfunction, and fibrotic disorders. Overexpression can enhance migration and invasion in certain tumors, while loss impairs cytoskeletal resilience. The Zyxin antibody is widely used to investigate its role in metastasis, cell adhesion signaling, and smooth muscle plasticity. Studies have also linked Zyxin to stretch-activated signaling in cardiac and vascular cells, highlighting its contribution to mechanobiology.
The Zyxin antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, producing distinct cytoplasmic and focal adhesion staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
