Ubiquitin-Proteasome System

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2050 products
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Proteinase K
Enzymes, Proteases Protein Purification, Enzyme Activity, Gene Expression
From $95.00
Regular price From $95.00
In Stock at Manufacturer
Proteinase K Solution (20 mg/mL)
Enzymes, Proteases Protein Purification, Enzyme Activity, Gene Expression
From $65.00
Regular price From $65.00
In Stock at Manufacturer
IHC image of CSB-RA912122A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Target: UBB
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Immunofluorescence staining of Hela cell with CSB-RA833619A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Target: UBB
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), A431 whole cell lysate(30µg)
All lanes: RNF7 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time:2min
Target: RNF7
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot, 
Positive WB detected in:Hela whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), K562 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), U251 whole cell lysate(30µg), A431 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg)
All lanes: UBE2B antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time:120s
Target: UBE2B
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: K562 whole cell lysate(30µg) ,Jurkat whole cell lysate(30µg) , NIH/3T3 whole cell lysate(30µg) 
All lanes: Cullin 1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time:2min
Target: CUL1
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: K562 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), A549 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg)
All lanes:UBE2L3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time:10s
Target: UBE2L3
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30µg) , U-251MG whole cell lysate(30µg) , Jurkat whole cell lysate(30µg) , K562 whole cell lysate(30µg) , HEK293 whole cell lysate(30µg) , HEK293T whole cell lysate(30µg) , SW620 whole cell lysate(30µg) 
All lanes: Parkin antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 30 kDa
Exposure time: 2min
Target: PRKN
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA145805A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Target: UBB
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg)
All lanes: RING2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time:2min
Target: RNF2
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot, 
Positive WB detected in:PC-3 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), K562 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), A431 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat Small intestine lysate(30µg)
All lanes: Cullin 2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time:120s
Target: CUL2
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Immunofluorescence staining of MCF-7 cell with CSB-RA036685A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Target: UBB
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: A549 whole cell lysate(30µg) , HeLa whole cell lysate(30µg) , HepG2 whole cell lysate(30µg) , HEK293 whole cell lysate(30µg) , K562 whole cell lysate(30µg) , SH-SY5Y whole cell lysate(30µg) , NIH/3T3 whole cell lysate(30µg) 
All lanes: UBE2I antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 3min
Target: UBE2I
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA012285A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Target: UBB/UBC
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Overlay Peak curve showing MCF7 cells stained with CSB-RA316735MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C.
Target: UBC
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration,  antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Target: UBE2C
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA980583A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.57% DAB.
Target: MDM2
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate
All lanes: RNF20 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa
Target: RNF20
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
From $210.00
Regular price From $210.00
In Stock at Manufacturer
IHC image of CSB-RA898115A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Target: CUL3
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Immunohistochemistry
From $210.00
Regular price From $210.00
In Stock at Manufacturer
No image
Target: RNF122
Antibodies, Primary Antibodies Western Blot
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: RNF122
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
IHC image of CSB-PA019825LA01HU diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Target: RNF122
Antibodies, Primary Antibodies ELISA, Immunohistochemistry, Immunofluorescence
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: RNF122
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: UBB
Antibodies, Primary Antibodies Western Blot
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: UBB
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
IHC image of CSB-PA04837A0Rb diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Target: UBB
Antibodies, Primary Antibodies ELISA, Immunohistochemistry
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: UBB
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: RNF2
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: UBE2M
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: RNF2
Antibodies, Primary Antibodies Western Blot
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: UBE2M
Antibodies, Primary Antibodies ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: MDM2
Primary Antibody ELISA
From $166.00
Regular price From $166.00
In Stock at Manufacturer
Western Blot
Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, LO2 whole cell lysate, Jurkat whole cell lysate
All lanes: UBC antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
Target: UBC
Antibodies, Primary Antibodies ELISA, Western Blot
From $166.00
Regular price From $166.00
In Stock at Manufacturer
MDM2 Antibody – Figure 1 of 2
Target: MDM2
Primary Antibody ELISA, Western Blot, Immunohistochemistry
From $166.00
Regular price From $166.00
In Stock at Manufacturer
No image
Target: MDM2
Primary Antibody Western Blot
From $166.00
Regular price From $166.00
In Stock at Manufacturer

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