| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids with a relative molecular weight of 29.3 kDa.The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide .Proteinase K is widely used for the preparation of chromosomal DNA , and removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines.Denaturants such as SDS (1%) can increase their activity.It is commonly used at working concentrations of 50-100 μg/mL.
Features
Enzyme activity ≥30 U/mg
No RNase residue
No DNase residue
Applications
Nucleic Acid Extraction & Purification
Remove endogenous nucleases during the preparation of DNA and RNA
Prepare tissue sections for in - situ hybridization.
Specifications
|
Cat.No. |
10412ES03 / 10412ES10 / 10412ES25 /10412ES76 |
|
Size |
1 mL /10 mL/ 25 mL / 500 mL |
Glycerol Content |
Contains Glycerol |
Shipping and Storage
This product should be stored at 2~8℃ for 2 years.For longer storage, it can be stored at -25~-15℃.
Product Properties
|
Source |
Yeast |
|
CAS NO. |
39450-01-6 |
|
Molecular Weight |
29 kD |
|
Units/mg |
≥30 U/mg |
|
Appearance |
Liquid |
Store this enzyme at 2~8°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Gel Pack and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Nucleic Acid Extraction & Purification Remove endogenous nucleases during the preparation of DNA and RNA Prepare tissue sections for in - situ hybridization.. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U/mg) is defined as the amount of protease activity per milligram of enzyme protein under standardized assay conditions (substrate concentration, pH, temperature, and incubation time).
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Most serine proteases (e.g., Proteinase K) are inhibited by PMSF (1 mM) and heat-inactivated at 95°C for 10 min. Proteinase K retains activity across a broad pH range (4–12) and tolerates SDS, urea, and other denaturants, making it particularly effective for nucleic acid extraction from challenging samples including FFPE tissue.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
