| Field | Specification |
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| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc |
| Shipping | |
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Overview
For quantitative determination of salicylate in biological samples and beauty products, mouthwash, etc. The assay uses OD560nm for signal readout. Compatible sample input includes Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD560nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 30 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Fast and sensitive. Linear detection range: 0.8 mM (10.9 mg/dL) to 20 mM (274.2 mg/dL) salicylate with 20 µL sample (96-well).
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent; High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of salicylate within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
SALICYLATEis a salt or ester of salicylic acid and can be found naturally in some plants. It is also a metabolic byproduct of aspirin (acetylsalicylic acid) and salicylate concentrations are often tested in blood or urine in cases of suspected overdose. Salicylic acid is commonly used in skincare products as an exfoliating ingredient and in other consumer products as a preservative. BioAssay Systems’ salicylate detection kit provides a convenient and reliable means to measure salicylate. In the assay, salicylate complexes with ferric chloride create a colored compound that can be measured at 560 nm. This assay can be used with a variety of samples and is simple, sensitive, and adaptable to high-throughput screening.
Detection method
Colorimetric (OD 560 nm).
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify salicylate in serum, plasma, urine by OD560 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma, urine handling.
- Monitor time-course or pre/post changes in serum, plasma, urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
