| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Storage |
Description
1×dsDNA HS Assay Kit is a rapid, highly sensitive and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit is highly selective for dsDNA and has good linearity in the range of 0.2 ng-100 ng, the quantitation range is between 10 pg/μL to 100 ng/μL. This kit is easy to operate, providing a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometer or Fluorescence Microplate Reader. It is ideal choice for NGS large-scale DNA sample quantification (such as input DNA quantification, DNA library quantification, etc.). This kit is well tolerated to common contaminants such as proteins and salts.
Features
- Fast: Ready-to-use premixed solution for easy handling; results available in just 5 minutes.
- Sensitive: Accurately quantifies samples ranging from 0.2 to 100 ng.
- Accurate: Minimal deviation compared to competing products, with all errors controlled within 10% and low batch-to-batch variation.
- Stable: Consistent performance across different production batches.
Components
|
Components No. |
Name |
Concentration |
12642ES60 (100 T) |
12642ES76 (500 T) |
|
12642-A |
1×dsDNA HS Working Solution |
1×Concentrate in DMSO |
50 mL |
250 mL |
|
12642-B |
dsDNA Standard 1 |
0 ng/μL in TE buffer |
1 mL |
|
|
12642-C |
dsDNA Standard 2 |
10 ng/μL in TE buffer |
1 mL |
5×1 mL |
Shipping and Storage
This product should be stored at 2~8℃ away from light for 12 months.
Application
dsDNA quantification; DNA concentration determination
Figures
1. Easy-to-use and streamlined quantification workflow
Figure 1. DNA Quantification Workflow
2. Good linearity: 12642 has good linearity in the range of 0.2-100 ng.
Figure 2. Linear Relationship Test
Linear quantification of 11 dsDNA concentrations (0.5–10 ng/μL) using 12642. Fluorescence was measured with Qubit Fluorometer 3.0. Results show good linearity across 0.2–100 ng total dsDNA.
3. Dye binding specificity
Figure 3. Specificity test
Test Scheme: DNA standard S2 (1–100 ng gradient: 1, 5, 10, 20, 40, 60, 80, 100 ng), along with equal-mass samples of ssDNA, RNA, dsDNA+ssDNA mix, and dsDNA+RNA mix were tested.
Conclusion: Adding equal amounts of ssDNA slightly increases the 12642 reading; error <10%(left). Adding equal amounts of RNA also increases the 12642 reading; error <10%(right).
4. Efficient dye binding and stable fluorescence signal
Figure 4. Dye binding efficiency and fluorescence signal persistence
Test Scheme: Two RNA libraries of different concentrations were measured using 12642 on the Qubit 3.0 with a 2 μL sample volume.
Conclusion: The fluorescence signal saturated within 2 minutes and remained stable for up to 5 h.
5. Strong tolerance to common contaminants
6. Ultra-high stability
Figure 5. Stability comparison of Yeasen's (Cat#12642ES) and competitor T brand
Test Scheme: Two dsDNA concentrations were measured over time using supplier T* (4°C), Yeasen#12642 (4°C), and Yeasen#12642 (room temperature, ~25°C). Deviation from the initial value (Day 0) was calculated.
Conclusion: Yeasen#12642 remained stable at room temperature for 2 weeks, with deviations <10%.
FAQ
Q: What is the difference between 12640 and 12642?
A: 12642 is a premixed solution, ready-to-use. It can be directly added to the sample to determine the concentration. There is no need to prepare a dye working solution.
Q: The product composition consists of only two standard substances. When creating the standard curve, is it necessary to perform multiple dilutions on the "standard 2" sample?
A: When using Qubit for quantification, only two standards are needed according to the operation steps. If using an enzyme reader, multiple gradients are required to create the standard curve. In this case, 1×TE can be used to gradientally dilute standard 2.
Q: If we replace Qubit detection with an enzyme detector, how should we select the enzyme detection plate and how should the wavelength be set?
A: Select the black microplate. Wavelength: Ex = 480 nm, Em = 520 nm.
Q: Is there a difference between the fluorescence value of the standard sample and the previous ones? What should the typical fluorescence value of a standard sample be?
A: The fluorescence value of the standard sample is not fixed, and there may be differences among different instruments. To verify, you can perform multiple replicate wells or use multiple gradients, and if a linear relationship is observed, it indicates success.
Documents:
Safety Data Sheet
Manuals
This kit requires a fluorometer capable of detecting the kit's excitation/emission wavelength (typically ~480/520 nm for dsDNA HS and similar dyes). The Qubit fluorometer (Thermo Fisher) or compatible fluorometer-based systems are recommended. Standard UV-Vis spectrophotometers using A260 cannot substitute for fluorometric quantification when sample purity or volume is limiting.
Sensitivity varies by kit format: the HS (High Sensitivity) kit detects dsDNA as low as 0.2 ng/µL and the BR (Broad Range) kit covers 2–1000 ng/µL. Refer to the specific product datasheet for the validated linear range. Samples exceeding the range must be diluted before measurement.
Yes. The HS and BR dsDNA kits contain intercalating dyes with strong preference for double-stranded DNA over RNA and ssDNA. This selectivity makes them suitable for quantifying genomic DNA, PCR products, and library preparations in the presence of residual RNA without a DNase or RNase pre-treatment step.
Yes. Fluorometric dsDNA quantification is the recommended method for NGS library quantification, as it measures only double-stranded library fragments and is unaffected by free adapter dimers or unincorporated nucleotides (unlike A260). For highest accuracy on sequencing libraries, combine with Bioanalyzer or TapeStation sizing.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
