| Field | Specification |
|---|---|
| Activity | |
| Alternative Names | 3R12Rα-PMTX |
| Concentration | |
| Form | Lyophilized |
| Formulation | |
| Gene ID | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Solubility | Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. |
| Source | Synthetic peptide |
| Species | |
| Storage | |
| Target |
Overview
3R12Rα-Pompilidotoxin is a research-grade protein/peptide reagent used in research settings. It is commonly applied as a tool reagent related to NaV Na+ channels biology and/or assay development. It is supplied in Lyophilized format to support flexible downstream use in RUO workflows. Researchers commonly pair it with applications such as Electrophysiology.
Key elements and design rationale
- Molecular identity: MW: 1586 Da, Formula: C71H124N24O17.
- Source / origin: Anoplius samariensis (Solitary wasp).
- Quality attributes: Purity: ≥98% (HPLC); Bioassay tested: Yes; Sterile / endotoxin-free: No.
Modifications
Leu13 - C-terminal amidation
When used as a biochemical or pharmacological tool, results are best interpreted relative to the experimental system (species, expression level, and assay readout) and with appropriate negative and competition-style controls where relevant. This product is intended for research use only.
Biological background
Pompilidotoxins (PMTX, α and β) are small peptides consisting of 13 amino acids.α-PMTX (#P-170) induces a unique pattern of repetitive action potentials previous seen with other neurotoxins and thus facilitate both excitatory and inhibitory synaptic transmission.Recently it was found that this toxin slows the Na+-channel inactivation, without changing the peak current-voltage relationship or the activation time course of the TTX-sensitive Na+ currents in the neuromuscular synapse of the lobster walking leg and in the rat trigeminal ganglion neurons2-4. Effective at concentrations of 10 nM in cultured rat cortical neurons5.3R12Rα-Pompilidotoxin (3R12Rα-PMTX) is a mutated form of the α-PMTX, originally isolated from the solitary wasp (Anoplius samariensis) venom1 and is a synthetic version of the peptide.3R12RKα-PMTX is mutated at position 3 as well as 12 with Lysine to Arginine transition, which enhanced its activity on voltage-gated Na+ channels 5-fold relative to α-PMTX1,3.
Research relevance and current trends
- Using high-specificity ligands, toxins, and engineered peptides to dissect closely related receptor/channel subtypes and signaling microdomains.
- Pairing labeled (e.g., fluorescent) proteins/peptides with advanced imaging to map surface expression, trafficking, and nanoscale organization.
- Increasing emphasis on reproducibility through standardized characterization (identity, purity, and lot QC) and transparent reporting of reagent attributes.
Common research applications
- Electrophysiology: commonly used to compare signal, binding, or functional readouts across conditions without implying a specific protocol.
Across these use cases, changes in signal or functional readout are generally interpreted as evidence of differences in target abundance, accessibility, or engagement, but alternative explanations (matrix effects, off-target interactions, or assay artifacts) should be considered.
Notes for experimental interpretation
- Assay context matters: binding assays, functional modulation, and detection workflows can yield different readouts even for the same target system.
- Target complexity: closely related family members, splice variants, and post-translational modifications can influence apparent specificity and potency.
- Matrix and sample effects: buffer composition, detergents, and biological matrices may alter stability or apparent activity; interpret with appropriate controls.
- Control concepts: include negative controls and orthogonal validation (e.g., genetic perturbation or alternative reagents) to support robust interpretation.
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Konno, K.
et al. (2000) Neurosci. Lett.285, 29.
Schiavon, E.
et al. (2010) FEBS J.277, 918.
Sahara, Y.
et al. (2000) Eur. J. Neurosci.12, 1961.
Konno, K.
et al. (2000) Neurosci. Lett.285, 29.
Konno, K.
et al. (1998) Biochem Biophys. Res. Commun.250, 612.
Harsch, A.
et al. (1998) Neurosci. Lett.252, 49.
Schiavon, E.
et al. (2010) FEBS J.277, 918.
Sahara, Y.
et al. (2000) Eur. J. Neurosci.12, 1961.
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