| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | Up to 50 mM in methanol. Centrifuge all product preparations before use (5,000 x g for 1 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: 3-amino-6-chloro-N-(diaminomethylidene)-5-(dimethylamino)pyrazine-2-carboxamide;hydrochloride.
- Also known as: DMA, Dimethyl amiloride
- CAS number: 1214-79-5
- Molecular formula: C8H13Cl2N7O.
- Purity: >99% (HPLC)
- Concentration: 1-100 µM.
- Form: Lyophilized powder.
- Solubility: Up to 50 mM in methanol. Centrifuge all product preparations before use (5,000 x g for 1 min).
- Reconstitution: Up to 50 mM in methanol. Centrifuge all product preparations before use (5,000 x g for 1 min).
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: TRPA1 channel, Na+/H+ and Na+/Ca2+ exchangers
- Source: Synthetic
- Activity: 5-(N,N-Dimethyl)amiloride hydrochloride blocks TRPA1 channels with an IC50 of 80 µM1.
- Alternative names: DMA, Dimethyl amiloride
Scientific background
5-(N,N-Dimethyl)amiloride hydrochloride (DMA) is an amiloride derivative that selectively inhibits Na+/H+ exchangers in cellular membranes, resulting in the retention of H+ ions and in the decrease of intracellular pH. Amiloride derivatives are commonly used for their diuretic and antihypertensive properties.Several studies have reported that DMA has positive effects in pancreatic β-cells on insulin release as a result of mis-regulation of intracellular pH and enhanced insulin secretion. Thus, amiloride derivatives are promising potential therapeutic agents for Type 2 Diabetes. In addition, positive effects on other cell functions have been demonstrated such as, cell membrane electrical activity, increase in intracellular Ca2+ and retention of K+1. DMA also enables glucose to induce time-dependent potentiation and enables it to occur in situations where it is normally absent1,2. Another study has suggested the possibility of DMA protection of the heart against post-resuscitation myocardial injury3.DMA also has the ability to inhibit the Na+/Ca2+ exchanger and to increase its currents in rat cardiomyocytes. Experimental trials have showed that DMA increases calcium sensitivity in normal and hypertrophied cardiomyocytes4.DMA also blocks TRPA1 channels5.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
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