| Field | Specification |
|---|---|
| Mfr No | |
| Species |
The 9L/lacZ cell line is a well-characterized rat gliosarcoma cell line commonly used in neurobiological and oncological research. Originally derived from a nitrosourea-induced rat brain tumor, this line has been engineered to express the lacZ gene, which encodes the enzyme β-galactosidase. This modification facilitates the tracing and studying of tumor cells in vivo, particularly useful in experiments involving tumor progression and metastasis. The expression of lacZ allows for the easy identification of these cells using X-gal staining, which turns the cells blue when they express β-galactosidase.
These cells exhibit aggressive tumor-forming capabilities when implanted in immunocompromised or syngeneic hosts, making them a robust model for studying brain cancer dynamics and testing therapeutic strategies against gliomas. Additionally, the 9L/lacZ cell line has been utilized in gene therapy trials, particularly in assessing the efficacy of suicide genes and other genetic interventions aimed at controlling tumor growth. This line is also pivotal in understanding the interactions between tumor cells and the host's immune system, thereby contributing insights into the complexities of tumor immunology.
—
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
10%
OFF
15%OFF
20%
OFF
$50
OFF
FREE
SAMPLE
