| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human AATF recombinant protein (Position: Q5-H552) was used as the immunogen for the AATF antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
AATF Antibody / Apoptosis-antagonizing transcription factor is a anti-AATF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, Nucleolus.
Key elements and design rationale
- Target: AATF
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, ELISA
Biological background
Functionally, AATF antibody identifies a 560-amino-acid protein that binds transcription factors such as TP53, ATF5, and RNA polymerase II components to modulate gene expression. AATF promotes cell cycle progression by repressing pro-apoptotic genes and enhancing DNA repair. It also plays roles in ribosome biogenesis, RNA processing, and chromatin organization.
The AATF gene is located on chromosome 17q11.2 and is widely expressed in proliferating cells, especially in brain, liver, and reproductive tissues. AATF activity ensures proper coordination between transcription, cell cycle checkpoints, and apoptosis resistance during stress conditions.
Pathologically, overexpression of AATF is observed in several cancers, including glioblastoma and leukemia, where it enhances cell survival and tumor progression. Conversely, loss of AATF sensitizes cells to apoptosis induced by DNA damage or oxidative stress. Research using AATF antibody supports studies in transcription regulation, apoptosis, and oncogenic signaling.
AATF antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect transcriptional regulators.
Structurally, Apoptosis-antagonizing transcription factor contains nuclear localization signals, basic leucine zipper-like motifs, and regions for protein-protein interaction with transcription complexes. This antibody supports analysis of AATF's role in balancing survival and death signaling pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
