| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ACE2 recombinant protein (Position: Q18-F805) was used as the immunogen for the ACE2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ACE2 Antibody / Angiotensin-converting enzyme 2 is a anti-ACE2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: ACE2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Angiotensin-converting enzyme 2 is expressed in the vascular endothelium, lungs, heart, kidneys, and intestinal epithelium. Its catalytic domain hydrolyzes angiotensin II to produce angiotensin (1-7), which signals through the Mas receptor to counteract vasoconstriction and fibrosis. The ACE2 antibody enables precise detection of this enzyme in tissues, helping define its role in vascular tone and inflammatory control. Loss or downregulation of ACE2 disrupts this protective axis, predisposing tissues to hypertension, oxidative stress, and organ injury.
In addition to its enzymatic role, Angiotensin-converting enzyme 2 serves as a functional receptor for several coronaviruses, including SARS-CoV and SARS-CoV-2. Viral spike proteins bind to the extracellular domain of ACE2 to mediate cell entry. The ACE2 antibody supports virology and pathogenesis studies by enabling analysis of receptor abundance and distribution across tissues. Mapping ACE2 expression helps clarify host susceptibility and disease severity patterns in respiratory and cardiovascular systems.
Beyond infection biology, ACE2 participates in glucose metabolism, heart remodeling, and kidney filtration. Experimental models show that enhancing ACE2 activity mitigates cardiac hypertrophy and diabetic nephropathy. The ACE2 antibody supports quantification of these protective effects and is frequently used to monitor therapeutic modulation of the renin-angiotensin system. Dysregulated ACE2 expression has also been linked to inflammatory lung injury and endothelial dysfunction.
The ACE2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence, producing clear membrane and cytoplasmic staining consistent with its localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
