| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ACVR2A recombinant protein (Position: A20-N138) was used as the immunogen for the ACVR2A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ACVR2A Antibody / Activin receptor type-2A is a anti-ACVR2A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: ACVR2A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
ACVR2A binds activin A, activin B, and growth differentiation factors such as GDF8 (myostatin) and GDF11, initiating phosphorylation of type I receptor partners (ALK4/ALK7). This triggers SMAD2 and SMAD3 activation and downstream transcriptional responses that regulate gene expression in target tissues. Co-localization studies show ACVR2A clustering with ALK4 at the plasma membrane and endosomal compartments, where ligand-induced receptor internalization fine-tunes signaling output.
Structurally, ACVR2A features an extracellular ligand-binding domain, a single transmembrane region, and an intracellular kinase domain responsible for catalytic activity. It belongs to the transforming growth factor beta receptor family of serine/threonine kinases, sharing sequence homology with ACVR2B. The receptor interacts with ligands and type I receptors to form heterotetrameric complexes required for signal propagation. Known ligands include activins, inhibins, GDF8, and GDF11, which influence muscle growth, reproductive physiology, and developmental patterning.
Functionally, ACVR2A mediates activin signaling involved in follicle-stimulating hormone regulation, gonadal development, and muscle differentiation. It also participates in metabolic control, modulating insulin sensitivity and energy expenditure. During embryogenesis, ACVR2A expression is essential for mesoderm formation, neural tube development, and left-right axis determination. In adult tissues, ACVR2A supports tissue regeneration and wound repair by activating SMAD-dependent transcriptional networks.
Dysregulation of ACVR2A signaling contributes to reproductive disorders, cancer, and muscle-wasting diseases. Loss-of-function mutations can impair fertility, while overactivation has been linked to tumor progression through transforming growth factor beta pathway cross-talk. In hepatocellular carcinoma, reduced ACVR2A expression correlates with increased proliferation and metastasis. Pathway associations include transforming growth factor beta, SMAD, and MAPK signaling networks, which coordinate growth and differentiation across cell types. Isoform-specific studies reveal that ACVR2A and its paralog ACVR2B exhibit distinct ligand affinities and tissue distributions, allowing fine-tuned activin signaling.
Immunohistochemical staining using ACVR2A antibody shows membrane and cytoplasmic localization in reproductive, hepatic, and neural tissues. The ACVR2A antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
