| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ADAM12 recombinant protein (Position: V31-K476) was used as the immunogen for the ADAM12 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ADAM12 Antibody / Disintegrin and metalloproteinase domain-containing protein 12 is a anti-ADAM12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ADAM12
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, ADAM12 antibody identifies a 907-amino-acid protein containing a metalloprotease domain, a disintegrin domain, a cysteine-rich region, and a transmembrane segment. ADAM12 catalyzes the cleavage of cell-surface proteins such as growth factors, cytokines, and adhesion molecules, regulating intercellular communication and matrix remodeling. It acts on substrates including insulin-like growth factor binding proteins (IGFBPs), shedding them from the membrane to modulate IGF signaling.
The ADAM12 gene is located on chromosome 10q26.2 and produces two major isoforms: the long transmembrane form (ADAM12-L) and a shorter secreted variant (ADAM12-S). ADAM12 participates in tissue remodeling, myogenesis, and angiogenesis, and its expression increases during wound healing and fibrosis. It interacts with integrins via its disintegrin domain, influencing cell adhesion and migration. In skeletal muscle development, ADAM12 promotes myoblast fusion and differentiation by remodeling extracellular components.
In cancer biology, ADAM12 is frequently upregulated in breast, liver, and colorectal tumors, where it enhances growth factor signaling and tumor invasiveness. Elevated expression correlates with poor prognosis and therapy resistance. ADAM12 also participates in transforming growth factor-beta (TGF-beta) signaling and epithelial-to-mesenchymal transition (EMT), promoting metastatic potential. In cardiovascular tissues, ADAM12 contributes to vascular smooth muscle cell migration and atherosclerotic remodeling.
ADAM12 antibody is widely used in developmental biology, oncology, and extracellular matrix research. It is suitable for immunoblotting, immunohistochemistry, and zymography to assess ADAM12 expression, localization, and enzymatic activity. This antibody aids in studying protease-mediated signal activation, tissue remodeling, and tumor invasion. It also supports investigations into muscle differentiation and fibrotic disease mechanisms.
Structurally, ADAM12 features a catalytic zinc-binding motif within its metalloprotease domain and multiple adhesion modules that facilitate interaction with integrins and extracellular matrix proteins. Its activity is regulated by pro-domain removal, phosphorylation, and glycosylation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
