| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ADAMTS1 recombinant protein (Position: H411-S967) was used as the immunogen for the ADAMTS1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ADAMTS1 Antibody / A disintegrin and metalloproteinase with thrombospondin motifs 1 is a anti-ADAMTS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: ADAMTS1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, ADAMTS1 antibody recognizes a multi-domain enzyme that cleaves proteoglycans such as aggrecan and versican, controlling tissue remodeling during development, wound healing, and pathological fibrosis. ADAMTS1 also regulates angiogenesis by inhibiting endothelial cell proliferation and migration, in part through sequestration of VEGF signaling. The enzyme is expressed in many tissues including heart, kidney, and placenta, and its upregulation occurs in response to inflammatory cytokines, mechanical stress, and injury. ADAMTS1 participates in ovulation, cardiac morphogenesis, and tumor microenvironment remodeling.
Mutations or dysregulated expression of ADAMTS1 contribute to cardiovascular disease, cancer progression, and arthritis. Studies show reduced ADAMTS1 expression enhances tumor growth by promoting vascularization, whereas overexpression suppresses angiogenesis and metastasis. In the vascular system, ADAMTS1 modulates smooth muscle cell migration and matrix elasticity, maintaining vessel wall integrity. ADAMTS1 antibody is used to evaluate expression patterns and enzymatic activity via immunohistochemistry, zymography, and ELISA assays.
The ADAMTS1 gene, located on chromosome 21q21.3, encodes a 967-amino acid secreted glycoprotein activated by furin cleavage. Post-translational modifications, including glycosylation and proteolytic processing, regulate its localization and activity in the extracellular matrix. Interaction with TIMPs (tissue inhibitors of metalloproteinases) and integrins helps fine-tune its catalytic balance. In development, ADAMTS1 is crucial for follicular rupture during ovulation, kidney branching morphogenesis, and skeletal formation.
By targeting a central ECM protease, the ADAMTS1 antibody enables in-depth study of extracellular remodeling and angiogenic signaling in physiology and pathology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
