| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human AKT1 + AKT2 + AKT3 was used as the immunogen for the AKT1/2/3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
AKT1/2/3 Antibody is a anti-AKT1/2/3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31A24 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: AKT1/2/3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31A24, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
AKT1/2/3 antibody is widely applied in cancer biology, metabolism research, and neuroscience. AKT1 regulates cell survival and growth, AKT2 controls glucose metabolism, and AKT3 influences brain development and function. Dysregulation of AKT signaling is a hallmark of many cancers, diabetes, and neurological diseases. By detecting all three isoforms, researchers can evaluate global AKT activity and isoform specific contributions.
Western blot assays detect AKT isoforms in diverse tissues, immunohistochemistry maps AKT distribution in tumors and metabolic organs, and immunofluorescence reveals cytoplasmic and membrane localization upon activation. ELISA provides quantitative measurement of AKT levels in clinical and experimental samples. These applications make AKT1/2/3 antibody a versatile reagent for broad pathway analysis.
AKT kinases regulate multiple downstream targets including mTOR, FOXO transcription factors, and GSK3. They promote protein synthesis, inhibit apoptosis, and modulate metabolism. Aberrant AKT signaling drives oncogenesis, drug resistance, and metabolic disorders. By applying AKT1/2/3 antibody, scientists can investigate how PI3K AKT pathways integrate signals from growth factors, nutrients, and stress.
Therapeutically, AKT inhibitors are under development for cancer and metabolic disease. Measuring AKT expression with antibody based assays provides biomarkers for drug development and patient stratification. AKT1/2/3 antibody therefore supports translational research linking kinase biology to clinical intervention.
AKT1/2/3 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
