| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | 5-Hydroxytryptamine receptor 2C, Serotonin receptor 2C, 5-HT2C |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
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| Target |
Overview
Anti-5HT2C Receptor (HTR2C) Antibody is an antibody targeting 5-Hydroxytryptamine receptor 2C, Serotonin receptor 2C, 5-HT2C Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IHC, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: 5-Hydroxytryptamine receptor 2C, Serotonin receptor 2C, 5-HT2C (also reported as 5-Hydroxytryptamine receptor 2C, Serotonin receptor 2C, 5-HT2C).
- Immunogen/epitope region: Extracellular, N-terminus.
- Homology note: Mouse- identical; human - 12 out of 14 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
5-hydroxytryptamine receptor 2C, 5HT2C/5HT2CR, also known as serotonin receptor 2C, is a transmembrane (TM) receptor subtype in the 5-HT2R family of class A G-protein coupled receptors (GPCRs). 5HT2CR is encoded by the HTR2C gene and mediates various physiological functions, including disease-related pathways and behaviors due to its effects on the central nervous system (CNS).1,2The 5HT2C receptor is composed of seven TM helices (TM I - VII), three extracellular (ECL 1-3) and three intracellular loops (ICL 1-3), an intracellular carboxy-terminus, and an extracellular amino-terminus. Activation of 5HT2CR through 5-HT binding to Gαq/11 triggers G protein-dependent signaling that activates phospholipase Cβ (PLCβ) and leads to phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis to produce inositol-1,4,5-trisphosphate (IP3), accumulation of the downstream IP3 metabolite inositol monophosphate (IP1), and diacylglycerol (DAG), resulting in increased levels of intracellular mobilization of Ca2+ and IP1.1Activation of 5HT2CR can regulate ion channels and transport processes, as well as activate other downstream effectors including phospholipase A2 (PLA2), phospholipase D (PLD), cyclic nucleotides, and extracellular signal-regulated kinases (ERK1/2).
Research relevance and current trends
- Mapping receptor/channel localization across neuronal subtypes and subcellular compartments.
- Linking trafficking or surface expression changes to activity-dependent signaling and plasticity.
- Using KO/KD or blocking-peptide concepts to strengthen antibody-based target assignment.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-SR034.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-SR034; Negative control: BLP-SR034.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
