| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody is an antibody targeting 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B Polyclonal raised in Rabbit (ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.). This antibody is commonly used in FC, IF, IHC to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B (also reported as 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B).
- Immunogen/epitope region: Extracellular, N-terminus.
- Homology note: Mouse - identicalHuman- not recommended for use with human samples (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Lot quality control (as provided): Western blot analysis (unlabeled antibody, #ASR-032), and immunohistochemistry (labeled antibody)..
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC. (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
5-Hydroxytryptamine receptor 3B (5-HT3B, HTR3B) belongs to the super-family of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the super-family of G-protein coupled receptors.The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B.
Research relevance and current trends
- Mapping receptor/channel localization across neuronal subtypes and subcellular compartments.
- Linking trafficking or surface expression changes to activity-dependent signaling and plasticity.
- Using KO/KD or blocking-peptide concepts to strengthen antibody-based target assignment.
Common research applications
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: RIC-001-AG.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-SR032; Negative control: RIC-001-AG.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
